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superscript iv first-strand cdna synthesis reaction kit  (Thermo Fisher)


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    Thermo Fisher superscript iv first-strand cdna synthesis reaction kit
    Superscript Iv First Strand Cdna Synthesis Reaction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iv+first-strand+cdna+synthesis+reaction+kit/high+capacity+cdna+reverse+transcription+kit/pm40523937-726-33-40
    Average 90 stars, based on 1 article reviews
    superscript iv first-strand cdna synthesis reaction kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Reconstruction of the molecular evolution of Usutu virus in Germany: Insights into virus emersion and circulation
    Article Snippet: .. In short, RNA was reverse transcribed using the SuperScript IV First-Strand cDNA Synthesis Reaction Kit (Cat. no. 18091050; Invitrogen by Thermo Fisher Scientific, Darmstadt, Germany) with random primers as previously described by Quick et al. 2017 [ ]. .. Followed by an USUV-specific multiplex PCR which was performed with two separate mixes of primer pairs using AccuPrime Taq DNA Polymerase High Fidelity (Cat. no. 12346–086; Invitrogen) [ ].

    Article Title: Reconstruction of the molecular evolution of Usutu virus in Germany: Insights into virus emersion and circulation.
    Article Snippet: .. In short, RNA was reverse transcribed using the SuperScript IV First-Strand cDNA Synthesis Reaction Kit (Cat. no. 18091050; Invitrogen by Thermo Fisher Scientific, Darmstadt, Germany) with random primers as previously described by Quick et al. 2017 [71]. .. Followed by an USUV-specific multiplex PCR which was performed with two separate mixes of primer pairs using AccuPrime Taq DNA Polymerase High Fidelity (Cat. no. 12346–086; Invitrogen) [72].

    Article Title: A TRUSTED targeted mass spectrometry assay for pan-herpesvirus protein detection
    Article Snippet: .. RNA was reverse-transcribed into cDNA using the SuperScript IV First-Strand cDNA Synthesis Reaction Kit (ThermoFisher Scientific, 18091050), following the manufacturer protocol. .. CPS1 cDNA abundance was quantified by qPCR (ViiA7 Real-Time PCR System) using the SYBR green PCR master mix (ThermoFisher Scientific, 4368706) and the following primers: CPS1 primer pair 1 (forward: 5′-AATGAGGTGGGCTTAAAGCAAG-3′; reverse: 5′-AGTTCCACTCCACAGTTCAGA-3′), CPS1 primer pair 2 (forward: 5′-ACTTCAGTTGAGTCCATTATGGC-3′; reverse: 5′-GGAACGGATCATCACTACTGGGTAG-3′), GAPDH primer pair (forward: 5′-TTCGACAGTCAGCCGCATCTTCTT-3′; reverse: 5′-CAGGCGCCCAATACGACCAAATC-3′).

    cDNA Synthesis:

    Article Title: Reconstruction of the molecular evolution of Usutu virus in Germany: Insights into virus emersion and circulation
    Article Snippet: .. In short, RNA was reverse transcribed using the SuperScript IV First-Strand cDNA Synthesis Reaction Kit (Cat. no. 18091050; Invitrogen by Thermo Fisher Scientific, Darmstadt, Germany) with random primers as previously described by Quick et al. 2017 [ ]. .. Followed by an USUV-specific multiplex PCR which was performed with two separate mixes of primer pairs using AccuPrime Taq DNA Polymerase High Fidelity (Cat. no. 12346–086; Invitrogen) [ ].

    Article Title: Forced MyD88 signaling in microglia impacts the production and survival of regenerated retinal neurons
    Article Snippet: RNA was quantified and quality checked using a NanoDropTM One Microvolume UV-Vis Spectrometer (Thermo Scientific). .. Following RNA extractions, cDNA was synthesized using SuperScript IV First-Strand cDNA Synthesis Reaction kit (Invitrogen) using random hexamers. cDNA samples were stored at −20°C until qPCR reaction set up. ..

    Article Title: Reconstruction of the molecular evolution of Usutu virus in Germany: Insights into virus emersion and circulation.
    Article Snippet: .. In short, RNA was reverse transcribed using the SuperScript IV First-Strand cDNA Synthesis Reaction Kit (Cat. no. 18091050; Invitrogen by Thermo Fisher Scientific, Darmstadt, Germany) with random primers as previously described by Quick et al. 2017 [71]. .. Followed by an USUV-specific multiplex PCR which was performed with two separate mixes of primer pairs using AccuPrime Taq DNA Polymerase High Fidelity (Cat. no. 12346–086; Invitrogen) [72].

    Article Title: Traumatic injury causes selective degeneration and TDP-43 mislocalization in human iPSC-derived C9orf72 -associated ALS/FTD motor neurons
    Article Snippet: .. 1-2μg of RNA was used as input for generation of cDNA according to the SuperScript IV First-Strand cDNA Synthesis Reaction Kit (Thermo Fisher Scientific). .. RT-PCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) on the CFX system (Bio-Rad).

    Article Title: ZNF280A links DNA double-strand break repair to human 22q11.2 distal deletion syndrome.
    Article Snippet: DNA double-strand breaks (DSB) are among the most deleterious forms of DNA damage and, if unresolved, result in DNA mutations and chromosomal aberrations that can cause disease, including cancer.. Repair of DSBs by homologous recombination requires extensive nucleolytic digestion of DNA ends in a process known as DNA-end resection.. In recent years, progress has been made in understanding how this process is initiated, but the later stages of this process—long-range DNA-end resection—are not well understood.

    Article Title: A TRUSTED targeted mass spectrometry assay for pan-herpesvirus protein detection
    Article Snippet: .. RNA was reverse-transcribed into cDNA using the SuperScript IV First-Strand cDNA Synthesis Reaction Kit (ThermoFisher Scientific, 18091050), following the manufacturer protocol. .. CPS1 cDNA abundance was quantified by qPCR (ViiA7 Real-Time PCR System) using the SYBR green PCR master mix (ThermoFisher Scientific, 4368706) and the following primers: CPS1 primer pair 1 (forward: 5′-AATGAGGTGGGCTTAAAGCAAG-3′; reverse: 5′-AGTTCCACTCCACAGTTCAGA-3′), CPS1 primer pair 2 (forward: 5′-ACTTCAGTTGAGTCCATTATGGC-3′; reverse: 5′-GGAACGGATCATCACTACTGGGTAG-3′), GAPDH primer pair (forward: 5′-TTCGACAGTCAGCCGCATCTTCTT-3′; reverse: 5′-CAGGCGCCCAATACGACCAAATC-3′).

    Synthesized:

    Article Title: Forced MyD88 signaling in microglia impacts the production and survival of regenerated retinal neurons
    Article Snippet: RNA was quantified and quality checked using a NanoDropTM One Microvolume UV-Vis Spectrometer (Thermo Scientific). .. Following RNA extractions, cDNA was synthesized using SuperScript IV First-Strand cDNA Synthesis Reaction kit (Invitrogen) using random hexamers. cDNA samples were stored at −20°C until qPCR reaction set up. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Forced MyD88 signaling in microglia impacts the production and survival of regenerated retinal neurons
    Article Snippet: RNA was quantified and quality checked using a NanoDropTM One Microvolume UV-Vis Spectrometer (Thermo Scientific). .. Following RNA extractions, cDNA was synthesized using SuperScript IV First-Strand cDNA Synthesis Reaction kit (Invitrogen) using random hexamers. cDNA samples were stored at −20°C until qPCR reaction set up. ..

    other:

    Article Title: The role of agricultural drainage, storm-events, and natural filtration on the biogeochemical cycling capacity of aquatic and sediment environments in Lake Erie's drainage basin.
    Article Snippet: Cleaned RNA was then converted into cDNA using the Invitrogen Superscript IV First-Strand cDNA Synthesis Reaction kit (Invitrogen), following the S. Knorr et al. Science of the Total Environment 905 (2023) 167102 manufacturer’s recommended protocol for random hexamers.

    RNA Extraction:

    Article Title: ZNF280A links DNA double-strand break repair to human 22q11.2 distal deletion syndrome.
    Article Snippet: DNA double-strand breaks (DSB) are among the most deleterious forms of DNA damage and, if unresolved, result in DNA mutations and chromosomal aberrations that can cause disease, including cancer.. Repair of DSBs by homologous recombination requires extensive nucleolytic digestion of DNA ends in a process known as DNA-end resection.. In recent years, progress has been made in understanding how this process is initiated, but the later stages of this process—long-range DNA-end resection—are not well understood.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: ZNF280A links DNA double-strand break repair to human 22q11.2 distal deletion syndrome.
    Article Snippet: DNA double-strand breaks (DSB) are among the most deleterious forms of DNA damage and, if unresolved, result in DNA mutations and chromosomal aberrations that can cause disease, including cancer.. Repair of DSBs by homologous recombination requires extensive nucleolytic digestion of DNA ends in a process known as DNA-end resection.. In recent years, progress has been made in understanding how this process is initiated, but the later stages of this process—long-range DNA-end resection—are not well understood.



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